Additional Selective and Differential Cell Culture Media
NEOGEN Hektoen Enteric (HE) Agar, 500 g
Hektoen Enteric Agar is used for the isolation and differentiation of enteric pathogens. Hektoen Enteric Agar is not intended for use in the diagnosis of disease or other conditions in humans. Hektoen Enteric Agar was developed in 1967 by King and Metzger. Compared to other enteric differentiating media commonly used, Hektoen Enteric Agar increased the isolation rate of Salmonella spp. and Shigella spp. This was accomplished by increasing the carbohydrate and peptone content of the medium in order to counteract the inhibitory effects of bile salts and indicators. King and Metzger formulated a medium that slightly inhibited growth of Salmonella and Shigella, while inhibiting Gram-positive microorganisms. Enzymatic Digest of Animal Tissue provides nitrogen, carbon, and amino acids required for organism growth.For more information visit https://www.neogen.com/solutions/Microbiology/hektoen-enteric-agar
NEOGEN Lauryl Tryptose (LST) Broth, 500 g
Lauryl Tryptose (LST) Broth is used for the detection of coliform bacteria in water and wastewater. Lauryl Tryptose Broth is not intended for use in the diagnosis of disease or other conditions in humans.Lauryl Tryptose Broth is a selective medium for the detection of coliforms in water, dairy products and other foods. The American Public Health Authority (APHA) recommend Lauryl Tryptose Broth for the Most Probable Number Presumptive Test of coliforms in waters, effluent or sewage and as a confirmation test of lactose fermentation with gas production from milk samples and for the detection of coliforms in foods.Lauryl Tryptose Broth is prepared according to the formulation of Mallmann and Darby. Mallmann and Darby showed that tryptose at a concentration of 2% increased the early logarithmic growth phase when compared to meat peptone.For more information visit https://www.neogen.com/solutions/Microbiology/lauryl-tryptose-broth
NEOGEN Lactobacilli MRS Agar, 500 g
Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-agar
NEOGEN M-Green Yeast and Fungi Broth, 500 g
M-Green Yeast and Fungi Broth is used for the detection of fungi in beverages in a laboratory setting. M-Green Yeast and Fungi Broth is not intended for use in the diagnosis of disease or other conditions in humans.M-Green Yeast and Fungi Broth is a relatively more complex formula compared to other media used for isolation of fungi and yeast. This formulation is rich in nutrients which allows for excellent fungal growth. Bacterial growth is inhibited by an acid pH.Fungi have been found in potable water and on the inner surface of distribution system pipes. They can survive water treatment or they enter the system after treatment and remain viable.For more information visit https://www.neogen.com/solutions/Microbiology/m-green-yeast-and-fungi-broth
VITRO BIOPHARMA Pancreatic Stellate CAFs Maintenance Medium, Serum Free, 500ml
MSC-GRO™ Pancreatic Cancer-Associated Fibroblast (CAF) Maintenance Medium provides the essential nutrients for the growth and expansion of our Human Pancreatic Adenocarcinoma Cancer-Associated Fibroblasts (CAFs) (Cat. No.: CAF08). It is a complete media that supports several passages of Pancreatic CAFs and decreases doubling time.
NEOGEN Tryptone Glucose Extract (TGE) Agar, 500 g
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-glucose-extract-tge-agar
STEMCELL Technologies PneumaCult™ Airway Organoid Kit, Size: 1 Kit
Serum- and BPE-free medium for efficient establishment and differentiation of airway organoids
NEOGEN KF Streptococcus Agar, 500 g
KF Streptococcus Agar is used with triphenyltetrazolium chloride for the selective isolation and enumeration of fecal streptococci in a laboratory setting. KF Streptococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.KF Streptococcus Agar was developed by Kenner, Clark, and Kabler for the detection of fecal streptococci in surface waters. KF Streptococcus Agar is currently used in detecting fecal streptococci in water, foods, and other materials through the membrane filtration technique or pour plate method. Fecal streptococci are normal inhabitants in the intestines, and the presence of fecal streptococci can be used as an indication of fecal contamination. The presence of fecal streptococci is of value in determining pollution sources because certain fecal streptococci are host-specific.For more information visit https://www.neogen.com/solutions/Microbiology/kf-streptococcus-agar
NEOGEN Brain Heart Infusion Agar, 500 g
Brain Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans.A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious organisms, the phosphate buffer will help neutralize the acids produced from the utilization of glucose and thus maintain viability. The medium is not recommended for the determination of hemolytic reactions because of the glucose content.
For more information visit https://www.neogen.com/solutions/Microbiology/brain-heart-infusion-agar
NEOGEN Sodium Chloride (Bacteriological), 500 g
Sodium Chloride is a salt often used when preparing microbiological culture media in a laboratory setting. Sodium Chloride is not intended for use in the diagnosis of disease or other conditions in humans. When added to culture media Sodium Chloride allows for adjustments in osmotic pressure.For more information visit https://www.neogen.com/solutions/Microbiology/sodium-chloride-bacteriological
NEOGEN Alkaline Saline Peptone Water (ASPW) (ISO), 500 g
Alkaline Saline Peptone Water is a medium for the enrichment of Vibrio spp. from food and water samples according to ISO 21872-1:2017 and ISO 21872-2:2007, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Shread, Donovan & Lee as an enrichment broth for Aeromonas spp. and identified by Cruickshank as an effective medium for the enrichment of Vibrio spp., Alkaline Saline Peptone Water uses elevated pH and salt levels to provide a favorable environment for the enrichment of Vibrio spp.Peptone provides essential vitamins, minerals, amino acids & nitrogen for growth requirements. Sodium chloride provides essential electrolytes for maintenance of the osmotic balance.Sub-culture onto Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar (NCM0052) will be required.For more information visit https://www.neogen.com/solutions/Microbiology/alkaline-saline-peptone-water-iso
NEOGEN A-1 Medium, 500 g
A-1 Medium is used for the detection of coliform organisms in water and food in a laboratory setting. A-1 Medium is not intended for use in the diagnosis of disease or other conditions in humans.Since the early 1900’s enumeration of coliform organisms, specifically E. coli, have been used to determine water purity. Elevated temperature and most-probable-number (MPN) methods are routinely used for analysis of water and food samples for presence of fecal coliforms. A-1 Medium was formulated to hasten E. coli recovery and reduce the incidence of false positive cultures.In 1972 Andrews and Presnell developed A-1 Medium. This medium recovers E. coli from estuarine water in 24 hours instead of 72 hours, and in greater numbers without the pre-enrichment step. A-1 Medium can be used in a single step procedure for the detection of fecal coliforms in source water, seawater, treated wastewater, and foods.For more information visit https://www.neogen.com/solutions/Microbiology/a-1-medium
NEOGEN Baird Parker Agar Base, 500 g
Baird Parker Agar is used for the cultivation of Staphylococcus aureus from foodstuffs according to ISO 6888, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally introduced in 1962, this medium was developed by Baird-Parker to overcome the problems of recovering damaged Staphylococcus aureus from foodstuffs. This version of the medium is formulated according to ISO 6888-1:1999+A1:2003 and is in compliance with ISO 6888-2:2003+A1:2003 and ISO 6888-3:2003.Baird-Parker medium is highly selective by nature, due to the presence of potassium tellurite and lithium chloride. Tellurite inhibits most coliforms and is also reduced to telluride by S. aureus, giving the typical black colonies. Glycine and sodium pyruvate are both used as growth factors by staphylococci while the pyruvate also neutralizes any toxic peroxides that may be formed.For more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar-base
NEOGEN Rappaport Vassiliadis Salmonella Enrichment Broth, 500 g
Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella spp. Conforms to Harmonized USP/EP/JP Requirements. Rappaport-Vassiliadis Salmonella Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopoeia for the enrichment and selective isolation of Salmonella spp.from non-sterile samples. Malachite green and magnesium chloride act as selective agents, combined with high osmotic pressure and low pH to effectively inhibit non-target competitive organisms. Soya peptone provides a source of nitrogen and potassium phosphate acts as a buffer. Some Salmonella (S. typhi and S. enteritidis) are known to be sensitive to malachite green and as such may fail to grow.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-salmonella-enrichment-broth
NEOGEN DNase Agar, 500 g
DNase Agar is used for the differentiation of microorganisms on the basis of deoxyribonuclease activity and is not intended for use in the diagnosis of disease or other conditions in humans.In 1956, Weckman and Catlin showed a correlation between increased DNase activity of Staphylococcus aureus and positive coagulase activity. Their research suggested DNase activity could be used to identify potentially pathogenic staphylococci. DiSalvo confirmed their results by obtaining excellent correlation between coagulase and DNase activity of staphylococci isolated from clinical specimens. Jeffries, Holtman, and Guse incorporated DNA in agar to study DNase production by bacteria and fungi. Polymerized DNA precipitates in the presence of 1N HCl, creating an opaque medium. Organisms that degrade DNA produce a clear zone around an inoculum streak.For more information visit https://www.neogen.com/solutions/Microbiology/dnase-agar